Characteristics and Whole-Genome Analysis of Limosilactobacillus fermentum Phage LFP02

Limosilactobacillus fermentum is a bacterium widely used in food production, medicine, and industrial fermentation. However, fermentation could fail due to phage contamination. L. bacteriophage LFP02 can be induced from IMAU 32579 using mitomycin C. To better understand the characteristics of this phage, its physiological genomic were evaluated. The results showed that optimal multiplicity infection was 0.01, burst size 148.03 ± 2.65 pfu/infective center. Compared temperature, pH had more obvious influence on viability, although adsorption capacity not affected by divalent cations (Ca2+ Mg2+) or chloramphenicol. Its genome 43,789 bp GC content 46.06%, including 53 functional proteins. other phages, chromosome deletion, insertion, inversion, which demonstrated it novel phage. This study expand knowledge biological bacteriophages provide some theoretical basis for prevention during